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The assay technique followed spss basic principle of competitive ELISA whereby there is competitors among spss help biotinylated and spss help non biotinylated antigen for spss help fixed choice of antibody binding sites. The amount of biotinylated antigen bound spss spss antibody is inversely proportional spss spss N acylserotonin attention of spss sample. When spss system is in equilibrium, spss free biotinylated antigen is removed by spss help washing step and spss antibody bound biotinylated antigen is decided by use of anti biotin alkaline phosphatase as marker and p nitrophenyl phosphate as substrate. Quantification of unknowns was achieved by comparing spss enzymatic undertaking of samples with spss help reaction curve arranged by using known criteria. Quantitative choice of GABA was done using ELISA immunoassay kit, spss help made of ALPCO. 300 L of diluted standards, controls and undiluted samples were placed into spss acceptable wells of spss extraction plate. Two washing cycles were carried out, after which 250 l elution buffer was placed into spss applicable wells of spss extraction plate, covered and shook followed by addition of 100 L of spss extract for subsequent derivatization. 10 L of NaOH was added spss all spss wells followed by 50 L of spss equalizing reagent fresh arranged before assay and shook for 1 min on spss help shaker 600 rpm. 600 rpm. 25 L of spss by-product was used for spss subsequent ELISA. Comparisons were made by spss one way ANOVA among spss manage and treated groups.